WebNov 18, 2011 · To test this hypothesis, we combined lymphocyte-restricted hCD2-Cre transgenic, Rosa26-YFP-Cre-reporter, and Dicer1 ‘floxed’ mice. In this model, 25–50% of Dicer1 wt/wt NK cells are YFP + marking expression of Cre. As expected, YFP+ NK cells from Dicer1 fl/fl and fl/wt mice were confirmed to excise Dicer1, ... WebWe engineered mice to have defective miRNA biogenesis (Dicer1 floxed) in a lymphocyte restricted fashion (hCD2-Cre). MiRNA-deficient NK cells have defective development and survival, manifest as many fewer NK cells in vivo. However, those NK cells that did develop appeared ‘hyperfunctional’ with exaggerated IFN-γ and degranluation ...

Tcf1 and Lef1 provide constant supervision to mature CD8+ T …

WebGeneration of hCD2-cre Transgenic Mice—The plasmid phCD2-cre, placing cre recombinase under the control of hCD2 promoter (21), was microinjected into mouse oocytes to obtain the transgenic mice. Generation of H19exI Mice—We used cre-loxP-based deletion strat-egy for generating the mutants (22). As shown in Fig. 1b, the targeting Web914 Targeting strategy Gene targeting strategy for B-hCD2 mice. The exons 1-4 of mouse Cd2 gene that encode the extracellular domain were replaced by human CD2 exons 1-4 in B-hCD2 mice. mRNA expression analysis Protein expression analysis Analysis of spleen immune cells Analysis of lymph node immune cells Analysis of blood immune cells pttston pa seafood

CXXC finger protein 1 is critical for T-cell intrathymic development ...

WebApr 17, 2015 · Two widely used transgenes for conditional gene recombination in hematopoietic cells are Vav-iCre driven from the mu-rine Vav1 promotor, and hCD2-iCre driven from the human CD2 promotor. … WebApr 17, 2015 · Two widely used transgenes for conditional gene recombination in hematopoietic cells are Vav-iCre driven from the murine Vav1 promotor, and hCD2-iCre … WebNational Center for Biotechnology Information ptu 5th sem syllabus