Cisplatin adducts

Author: lohl

August undefined, 2024

WebChromatin will also contain drug-adducts on proteins and these will not be recognized at all. Most people doing immunohistochemical techniques for DNA modifications … WebIn addition, defects in the mismatch-repair proteins hMSH6 and hMLH1 led to increased replicative bypass of cisplatin adducts, but not of oxaliplatin adducts. Finally, primer extension assays performed in the presence of HMG1, which is known to recognize cisplatin-damaged DNA, revealed that inhibition of translesion synthesis by HMG1 also ...

Recognition of cisplatin adducts by cellular proteins

WebJan 5, 2024 · Platinum-based compounds are highly potent anti-tumor drugs in a variety of cancer types, acting through the formation of covalent cisplatin-DNA adducts 3, 4, 5. These compounds exhibit only... WebIn general, combination of cisplatin with an antimetabolite, taxane, or topoisomerase inhibitor, can result in a modulation of platinum pharmacology on the DNA, for example, enhanced retention of the platinum-DNA adducts. These interactions are mostly sequence and cell type dependent. sharon lawton coaching

Recognition and repair of DNA-cisplatin adducts - PubMed

WebMay 8, 2014 · The masses of the platinum adducts following digestion and separation by HPLC were measured by mass spectrometry. Our results demonstrate that hydrated electrons induce damage to thymines as well as detachment of the cisplatin moiety from both guanines in the oligonucleotide. WebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by … pop up cards templates and directions

Oleuropein Ameliorates Cisplatin-induced Hematological Damages …

Cisplatin induced Mitochondrial DNA Damage In Dorsal Root …

WebApr 10, 2024 · Triple-negative breast cancer (TNBC) is one of the most aggressive forms of breast cancer and constitutes 10–20% of all breast cancer cases. Even though platinum-based drugs such as cisplatin and carboplatin are effective in TNBC patients, their toxicity and development of cancer drug resistance often hamper their clinical use. Hence, novel … WebDec 19, 2024 · For instance, cisplatin forms mainly 1,2-intrastrand crosslinks (~80%) and rarely interstrand crosslinks (~1.5%) between two nucleobases in double-stranded DNA [21,22], whereas transplatin can hardly form intrastrand crosslinks but forms monofunctional adducts and interstrand crosslinks at a higher frequency than cisplatin. pop up cards with flowersWebSep 5, 2011 · The recognition of inter- and intra-strand DNA adducts and the consequent generation of an apoptotic signal is often impaired in cisplatin-resistant cancer cells … pop up card with butterflies

"WebCisplatin is mainly eliminated via urine. The highest concentrations can be found in the liver, prostate and kidneys. Side effects include severe nausea, vomiting, ototoxicity and nephrotoxicity, together with myelosuppression (bone marrow suppression). " - Cisplatin adducts

Cisplatin adducts

Cisplatin induced Mitochondrial DNA Damage In Dorsal Root …

WebDespite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major phenolic compound and used as a possible natural antioxidant, antimicrobial, and anticancer agent. ... adducts. The treatment with cisplatin elevated the TOS and 8-OH-dG levels ... WebApr 9, 1999 · Cisplatin is well known as a DNA-damaging agent, and the specific adducts produced in DNA have been well characterized. However, the mechanism by which …

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WebCisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. ... DDP binds to DNA and forms DNA-platinum adducts, which disrupt DNA synthesis and ... WebThere was also a statistically significant inverse correlation between cisplatin/DNA adduct formation and the IC50 for cisplatin in these cell lines. (P = 0.0004, R2 = 0.67). In …

WebJan 1, 2001 · Pt adducts on DNA extracted from cells that had been treated with cisplatin were less immunoreactive than DNA treated with cisplatin in vitro, but after heating the immunoreactivity increased such that 50% inhibition in the assay was caused by 2 … WebFeb 28, 2024 · One of the main problems in chemotherapy using platinum drugs as anticancer agents is the resistance phenomenon. Synthesizing and evaluating valid alternative compounds is challenging. This review focuses on the last two years of progress in the studies of platinum (II)- and platinum (IV)-based anticancer complexes. In …

WebA) The cisplatin activation process occurs by exchange of one or two of its chlorides for water molecules (monoaquated and diaquated, respectively). B) Cisplatin can form covalent bonds with... WebThe cytotoxicity of platinum compounds is thought to be determined primarily by their DNA adducts. Cisplatin and oxaliplatin are structurally distinct, but form the same types of …

WebMar 29, 2006 · Here we describe a new method to establish and characterize monoclonal antibodies (Mab) for structurally defined DNA adducts. The two major reaction products of cisplatin, the guanine-guanine (Pt- [GG]) and adenine-guanine (Pt- [AG]) intrastrand crosslinks are recognized by Mab R-C18 and R-B3, respectively.

WebCisplatin is administered to cancer patients intravenously as a sterile saline solution (that is, containing salt—specifically, sodium chloride). Once cisplatin is in the … pop up cards to buyWebThe cytotoxicity of platinum compounds is thought to be determined primarily by their DNA adducts. Cisplatin and oxaliplatin are structurally distinct, but form the same types of adducts at the same sites on DNA. However, the DNA adducts are differentially recognized by a number of cellular proteins … pop up carnivals near meWebDespite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major … pop up carportsWebCisplatin is a chemotherapy medication used to treat a number of cancers. These include testicular cancer, ovarian cancer, cervical cancer, bladder cancer, head and neck … sharon law thompson engineeringWebMay 7, 2024 · The formation of adducts between cisplatin and nuclear DNA is not the only mechanism by which cisplatin induces cytotoxicity, and cisplatin also has significant toxicity to enucleated cells.[6 7] Some studies have shown that oxidative stress is related to cisplatin-induced cytotoxicity, and cisplatin induces intracellular reactive oxygen ... sharon lawton obituaryWebCisplatin is an effective, widely used drug for many cancers, which shows a high level and broad spectrum of antitumor activities. (1−6) It has been known that the binding between this drug molecule and the DNA molecule disables the physiologic activities of DNA and eventually induces cell apoptosis. pop up catcherWebJul 18, 2007 · One potential predictive marker is the formation of cisplatin-DNA adducts, which are formed when cisplatin reacts with the cellular DNA by binding to nucleotides. … pop up cat cards how to make easy